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FETAL BOVINE SERUM
LOW IgG

Cell Culture Tests
Growth Promotion-Acid Phosphatase Activity

Growth promotion shows the ability of the fetal bovine serum to support the growth of a cell line using a standardized low inoculum in media with a 10% serum over a period of 10 to 14 days.

The acid phosphatase assay is based on the conversion of p-nitrophenol-phosphate to p=nitrophenol by cytosolic acid phosphatase. The absorbance of p-nitrophenol at 405 nm is monitored as a measure of cell numbers. The growth data are expressed as the average absorbance relative to an internal validated standard.

Physical and Chemical Analysis Specifications Results

Optical Appearance Amber Liquid Complies    
pH (By Potentiometric Determination 7.0-7.5 7.1
Osmolality (By freeze-point Depression) 280-340 mosmol/kg 322 mosmol/kg
Endotoxin (By Kinetic-Turbidimetric LAL-assay) <10 EU/mL 7.4 EU/mL
Hemoglobin (By Spectophotometric Method) <20 mg/dL 4.2 mg/dL
Protein Specifications Results

Total Protein (By Biureth Method) 3.0-4.5 g/dL 3.7 g/dL
Albumin (By Electrophoresis) 55-75TP 58.4%TP
a-Globulin (By Electrophoresis) 5-25TP 22.8%TP
B-Globulin (By Electrophoresis) 6-25TP 17.2%TP
y-Globulin (By Electrophoresis) <1TP 0.5%TP
IgG Content (By Elisa) <1.5 ug/mL 1.1 ug/mL
Micro-Organism Testing Specifications Results

Aerobic Bacteria NOT DETECTED NOT DETECTED
Anaerobic Bacteria NOT DETECTED NOT DETECTED
Fungi NOT DETECTED NOT DETECTED
Mycoplasma NOT DETECTED NOT DETECTED
Virus Testing Specifications Results

Bovine Virus Diarrhoea (BVD-V) NOT DETECTED NOT DETECTED
Parainfluenza Type-3 (PI-3) NOT DETECTED NOT DETECTED
Infectious Bovine Rhinotracheitis (IBR) NOT DETECTED NOT DETECTED
Serum Antibody Titer Specifications Results

BVD-AB-1 (By Serum Neutralization Test) VALUE NEGATIVE
BVD-AB-2 (By Serum Neutralization Test) VALUE NEGATIVE

Call Today to Order Fetal Bovine Serum LOW IgG at 800-845-1445 Catalog Number: 106 Lot Number: 106724

 

 

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