FETAL BOVINE SERUM
EMBRYONIC STEM CELL TESTED
This fetal bovine serum is US origin and tested for the ability to support undifferentiated
morphology of embryonic stem cells (ESC’s).
ESC’s are isolated from the inner mass of the blastocyst
stage of development. These ESC’s are maintained in
vitro and tested pluripotency by transplanting back into an
adult to see if it forms a teratoma i.e. a tumor with from all the
germ layers (ectoderm, mesoderm and endoderm).
For human ESC’s, testing is by transplanting into a mouse
without an immune system and observing for the formation of an embryo-like
structure with all three germ layers.
It has been shown that after 600 population doublings (about three
years of maintenance), ESC’s continue to proliferate without
apparent problems. In fact, Scientists believe that ESC’s
can be grown in the laboratory in a pluripotent state indefinitely.
Ectoderm: Gives rise to skin, brain and nerves
Mesoderm: Becomes blood, heart, bone, kidney, muscle and
Endoderm: Develops into lung, liver and the digestive system
Pluripotent: Stems cells that make all cell types found in
an embryo, fetus or developed organism, except the trophoblast and
placenta. ESC’s are considered pluripotent.
Fetal Bovine Serum ES Qualification Assay
Serum ES Qualification assay: mouse embryonic stem cells are grown
for 2 passages in a complete medium supplemented with 15%, 20% and
30% fetal bovine serum. The bovine serum is scored based on factors which
include the size, morphology, differentiation state, plating efficiency
and feeder morphology at all three serum concentrations and compared
to an inhouse previously ES qualified serum.
Call Today to Order 559-688-0018 or 800-845-1445 Catalog Number:
104 Lot Number: 104300